Recombinant salmon FGF-2 protein 154 aa (bFGF/basic FGF) for the development of optimized serum-free culture media for species-specific Atlantic salmon (Salmo salar) in cellular agriculture protocols and veterinary research applications. This longer form of FGF-2 is used in comparative cell culture media optimization studies alongside Qk102, the 145aa form of salmon FGF-2.
FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary salmon cell culture. Receptor binding affinity and efficacy may differ depending on each species. Using a species-specific growth factor enhances receptor binding affinity, resulting in a lower concentration required in culture.
Salmon FGF-2 is a high purity 17 kDa FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).
- High purity salmon protein (UniProt number: XP_014067501.1)
- >98%, by SDS-PAGE quantitative densitometry
- Source: Expressed in E. coli
- 17 kDa (Monomer)
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilised from Tris/NaCl/CyS/mannitol
- Resuspend in water at >100µg/mL, prepare single-use aliquots, add carrier protein if desired, and store frozen at -20°C or -80°C