Recombinant Human Interleukin 3 (IL-3) is a pleiotropic cytokine which plays a crucial role in hematopoiesis and acts as a multi-lineage growth factor. As a hematopoietic growth factor, it promotes the proliferation, differentiation, and survival of various hematopoietic stem cells towards myeloid progenitors. IL-3 is commonly used in cell culture to stimulate the differentiation and maturation of human induced pluripotent stem cells towards mast cells, basophils, neutrophils, eosinophils, monocytes, and megakaryocytes.
Human IL-3 comprises 140 amino acids with a molecular weight of 15.2 kDa. This protein is animal origin-free, carrier protein-free, and tag-free to ensure a homogenous population with exceptional lot-to-lot consistency. IL-3 is suitable for reproducible and high-quality myeloid progenitors and more specific lineages.
- Highly pure human protein (UniProt number: P08700)
- >98%, by SDS-PAGE quantitative densitometry
- Source: Expressed in E. coli
- 15.2 kDa monomer
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilised from acetonitrile/TFA
- Resuspend in 10mM HCl, prepare single-use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C