Recombinant human vascular endothelial growth factor 165 (VEGF165/ VEGF-165/ VEGF165) protein is widely used in culturing primary endothelial cells, such as human umbilical vein endothelial cells (HUVEC).
VEGF165 is commonly used with human-induced pluripotent stem cells or embryonic stem cells-derived endothelial cells for developing human vascular tissue models. It has many applications including its use in neural research involving oligodendrocyte precursor cells, Schwann cells, astrocytes, and microglia. It plays a role in bone formation, regulates mesenchymal stem cell differentiation, and serves as a survival factor for chondrocytes, hematopoietic stem cells, and tumour cells.
Qkine recombinant human VEGF165 protein is a highly pure and potent 38 kDa homodimer, disulphide-linked consisting of two 165 amino acid polypeptide chains. It is an animal origin-free (AOF) and carrier protein-free (CF).
- High purity human VEGF165 (Uniprot: P15692)
- >98%, by SDS-PAGE quantitative densitometry
- 38.3 kDa (dimer), 19 kDa monomer
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilised from acetonitrile/TFA
- Resuspend in water at >100µg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C (short-term) or -80°C (long-term)