Follistatin-resistant activin A (FRACTA) protein has been engineered to prevent binding to the natural inhibitor, follistatin. In vivo activin A activity is regulated by follistatin, a high-affinity inhibitor; follistatin accumulates in stem cell culture, where it inhibits activin A.
Qk035 follistatin-resistant activin A (FRACTA) has equivalent bioactivity to wild-type activin A (Qk001) but does not bind follistatin so is resistant to feedback inhibition. High purity 26 kDa dimer comprising engineered mature domain of activin A protein, animal origin-free (AOF) and carrier-protein free (CF).
- High purity mature domain of follistatin-resistant activin A protein
- >98%, by SDS-PAGE quantitative densitometry
- 26 kDa (dimer)
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in our Cambridge, UK laboratories
- Lyophilised from acetonitrile, TFA
- Resuspend in 10mM HCl at >100µg/mL (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C