Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitised with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans. In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing "preservative carryover". Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).

• Analyte: non-selective
• Platform: dehydrated culture media
• Formula: enzymatic digest of casein 15.0g/L, enzymatic digest of soybean meal 5.0g/L, sodium chloride 5.0g/L, lecithin 0.7g/L, Tween 80 5.0g/L, agar 20.5g/L
• Grams per litre: 51.20
• Litres per 500g: 11.00
• Plates per 500g: 600.00