D/E Neutralising Agar with Tween is used for the isolation of microorganisms from sanitised environmental surfaces and is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralising Agar with Tween was developed by Dey and Engley to neutralise a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralising media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycolate media, and Neutralising Buffer.

Total neutralisation of disinfectants is critical. Disinfectant residues can result in a false negative (nogrowth) test. D/E Neutralising Agar effectively neutralises the inhibitory action of disinfectant carryover, allowing differentiation between bacteriostasis and true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralising Agar with Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.

• Analyte: non-selective
• Platform: dehydrated culture media
• Formula: enzymatic digest of casein 5.0g/L, yeast extract 2.5g/L, dextrose 10.0g/L, sodium thioglycolate 1.0g/L, sodium thiosulfate 6.0g/L, sodium bisulfite 2.5g/L, polysorbate 80 (Tween) 5.0g/L, lecithin 7.0g/L, bromcresol purple 0.02g/L, agar 15.0g/L
• Grams per litre: 54.00
• Litres per 500g: 9.00
• Plates per 500g: 500.00