Sugar Free Agar is used for the enumeration of psychrotrophic and mesophilic Gram-negative rods, and is not intended for use in the diagnosis of disease or other conditions in humans. A formula described by the International Dairy Federation for the enumeration of psychrotrophic and mesophilic Gram negative rods in butter and other dairy products. The Gram negative rods are able to deaminate proteins as a carbon source, whilst some enterococci are inhibited by this formula. The medium conforms to the formulation of the International Dairy Federation (I.D.F.).

• Analyte: bacillus, coliforms, E. coli, pseudomonas spp.
• Platform: dehydrated culture media
• Formula: gelatin peptone 7.5g/L, tryptone 7.5g/L, sodium chloride 5.0g/L, agar 14.0g/L
• Grams per litre: 34.00
• Litres per 500g: 15.00
• Plates per 500g: 800.00