Columbia Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Broth is not intended for use in the diagnosis of disease or other conditions in humans. Columbia Broth is prepared according to the formula described by Morello and Ellner. During their study, Columbia Broth was developed as a medium for blood cultures and found superior to a commonly used broth for growth of Staphylococcus aureus, E. coli, and streptococci.

• Analyte: non-selective
• Platform: dehydrated culture media
• Formula: enzymatic digest of casein 5.0g/L, enzymatic digest of animal tissue 5.0g/L, yeast extract 10.0g/L, enzymatic digest of heart muscle 3.0g/L, sodium chloride 5.0g/L, dextrose 2.5g/L, L-cystine 0.1g/L, magnesium sulfate 0.1g/L, ferrous sulfate 0.02g/L, tris (hydroxymethyl) aminomethane 0.83g/L, tris (hydroxymethyl) aminomethane-HCl 2.86g/L, sodium carbonate 0.6g/L
• Grams per litre: 35.00
• Litres per 500g: 14.00
• Plates per 500g: 780.00