MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonised USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonised USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonised USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonised USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.

• Analyte: E. coli
• Platform: dehydrated culture media
• Formula: pancreatic digest of gelatin 20.0g/L, lactose monohydrate 10.0g/L, dehydrated ox bile 5.0g/L, bromocresol purple 0.01g/L
• Grams per litre: 35.00
• Litres per 500g: 10.00