Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans. Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyse the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

• Analyte: Listeria spp.
• Platform: dehydrated culture media
• Formula: enzymatic digest of animal tissues 5.0g/L, enzymatic digest of casein 5.0g/L, meat extract 5.0g/L, yeast extract 5.0g/L, esculin 1.0g/L, disodium hydrogen phosphate 9.6g/L (equivalent to disodium hydrogen phosphate dihydrate 12.0g/L), potassium dihydrogen phosphate 1.35g/L, sodium chloride 20.0g/L, lithium chloride 3.0g/L, acriflavine 0.025g/L, nalidixic acid 0.02g/L
• Grams per litre: 55.00
• Litres per 500g: 9.00