Tryptic Soy Broth, Modified with Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. This medium conforms to USDA Formulation with Acid Digest of Casein.

The first major outbreak of E. coli O157:H7 was in 1982 and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurised milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

• Analyte: Enterohemorrhagic, E. coli, E. coli (EHEC)
• Platform: dehydrated culture media
• Formula: enzymatic digest of gelatin 17.0g/L, acid digest of casein 10.0g/L, sodium chloride 5.0g/L, dipotassium phosphate 4.0g/L, enzymatic digest of soybean meal 3.0g/L, dextrose 2.5g/L, bile salts no. 3 1.5g/L
• Grams per litre: 43
• Litres per 500g: 12