Application

Calcium chloride dihydrate has been used:to prepare competent E. coli for transformation(118) in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Packaging

100, 250, 500 g in poly bottle