Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.