Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Biochem/physiol Actions

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget’;s disease of bone (PDB).

Citation

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).3. Metcalf, D., Blood, 67, 257 (1986).4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

General description

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Packaging

10 µg in poly bottle

Physical form

Lyophilized from a 0.2 µm filtered solution of 1x PBS.