Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Biochem/physiol Actions

HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.

General description

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Packaging

10 µg in poly bottle

Physical form

Lyophilized from a 0.2 µm filtered solution of 1x PBS.