Bone Morphogenetic Protein 2 human, BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

Code: H4791-10UG D2-231

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondro...


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£312.00 10UG
£374.40 inc. VAT

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).

Biochem/physiol Actions

It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.

General description

Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Packaging

10 µg in poly bottle

Physical form

Lyophilized from a 0.2 µm filtered solution of 2x PBS + 6% Ethanol.

Preparation Note

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.

biological sourcehuman
formlyophilized powder
Gene Informationhuman ... BMP2(650)
impurities≤1 EU/µg Endotoxin level
mol wtdimer 30-38 kDa (glycosylated)
packagingpkg of 10 µg
potency≤60 ng/mL EC50
Quality Level100
qualityendotoxin tested
recombinantexpressed in HEK 293 cells
storage conditionavoid repeated freeze/thaw cycles
storage temp.−20°C
technique(s)cell culture | mammalian: suitable
UniProt accession no.P12643
Pack10UG
This product has met the following criteria: