Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

Physical form

Solution, 0.2 µm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant