Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPESbuffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.

General description

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.