Application

The ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.

Components

ES2N Basal Medium: (Cat. No. SCM083) One (1) 245 mL bottle containing standard base medium. Neuro27 Medium Supplement: (Cat. No. SCM013-S) One (1) 5 mL vial containing neural supplement (use as 50x). Neuro2 Medium Supplement: (Cat. No. SCM012-S) One (1) 1.25 mL vial containing N2-like supplement (use as 200x).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells into neurons. When used in conjucture with ESGRO Complete Plus medium mouse ES and iPS cells readily differentiate into neurons in a monolayer assay within 9-12 days on gelatin coated culture dishes. When supplemented with bFGF and EGF, this medium enables the isolation and expansion of neural stem cells (NSCs).

Quality

Cell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.