Application

Please see data sheet for detailed directions on Thawing, Plating , Feeding, Passaging, etc.

EndoGRO HUVEC should be cultured in EndoGRO medium (warmed to 37°C) for best results. Please see specific media data sheets for further information (SCME001).

The EndoGRO Human Umbilical Vein Endothelial Cells (HUVEC) are a commonly studied human cell type extracted from human neonatal umbilical cords.

Research CategoryStem Cell Research

Cell Line Description

Endothelial Cells

Cell Line Origin

Umbilical Cord

Components

SCCE001

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Human Neonatal Umbilical Cords

EndoGRO Human Umbilical Vein Endothelial Cells (HUVEC) are a commonly studied human cell type extracted from human neonatal umbilical cords. They are relatively easy to culture, and provide a valuable cell model for many vascular biology research applications, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction, and vasodilation. EndoGRO^® HUVEC are provided at passage 1, and have been quality tested in low-serum media without exposure to phenol red.

This product is for Research Use Only. This product is not approved for human or veterinary use, or for use in in vitro diagnostic or clinical procedures

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

ENDOGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500,000 cells

Quality

Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.

Further growth: Guaranteed to provide 15 or more doublings when grown in the appropriate EndoGRO Medium.

Storage and Stability

EndoGRO HUVEC are sold as cryopreserved ampoules. To maintain the cells' integrity, unpack the products immediately upon receipt. The cryopreserved cells should be placed in the liquid or gas phase of a liquid nitrogen dewar for storage.

Long-term storage of the ampoule must be in a liquid nitrogen dewar or a mechanical freezer designed for cryopreserved cell storage that maintains a temperature below -135°C.

Millipore recommends storing the cryopreserved vials in liquid nitrogen vapor phase. Handle cryopreserved vials with caution. Always wear eye protection and gloves when working with cell cultures. Aseptically vent any nitrogen from cryopreserved vials in a biosafety cabinet prior to thawing the vials in a water bath.