Analysis Note

ControlRNcell CX neural stem cells (SCC007).

Application

Anti-Nestin Antibody, clone 10C2, Alexa Fluor 488 conjugate detects level of Nestin & has been published & validated for use in ICC.

Research Sub CategoryNeurofilament & Neuron MetabolismNeuronal & Glial Markers

Research CategoryStem Cell ResearchNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Immunogen

Fusion protein corresponding to human Nestin.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).

Specificity

This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa