Analysis Note

ControlNTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Application

Immunofluorescence: A previous lot was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Purified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.

Format: Purified

Specificity

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Storage and Stability

Stable for 1 year at from date of receipt.

Target description

235/410 kDa