Analysis Note

ControlMouse embryonic stem cells (SCR012).

Application

Research CategoryStem Cell Research

Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.

Research Sub CategoryPluripotent & Early DifferentiationGlycobiology

Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen

F9 teratocarcinoma stem cells (X-irradiated).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium sulfate precipitation followed by size exclusion.

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).

Specificity

This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa