Application

HAT Media Supplement (50×) Hybri-Max^™ has been used:in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clonesin the generation of mAbs (monoclonal antibodies)in the luciferase-based bioassay (LUC) assay

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 µM hypoxanthine, 0.4 µM aminopterin, 16 µM thymidine.