Application

Research CategoryNeuroscience

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: E. coli

Nerve Growth Factor (β-NGF) is a well characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on nonneuronal tissues. β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.

Physical form

Recombinant animal free human β-NGF is manufactured using all non-animal reagents

Product is filtered through a 0.2 micron filter before lyophilization.

Quality

Bioactivity assay: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Specificity

Cross ReactivtyChicken, Hamster, Mouse, Rat

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.