Biochem/physiol Actions

The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

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General description

Human Hepatocyte Growth Factor (HGF) is also known as Scatter Factor and Hepatopoeitin A. HGF is a potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive.

GF116 is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (234 amino acids).

Product Source: Baculovirus infected High-5 cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

The sterile filtered solution was lyophilized from 0.1M L-arginine HCl, 10 mM Tris, pH 8.0, and 200 mM NaCl.

Storage and Stability

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted HGF should be stored in working aliquots at -20°C. We recommend a quick centrifugation of the vial followed by reconstitution in water to a concentration of 0.5 mg/mL. This solution can then be diluted into other buffers and stored at 4°C for 1 week or stored at -20°C for future use. Avoid repeated freeze/thaw cycles.