Biochem/physiol Actions

Biglycan interacts with collagen type I and II, fibronectin and TGF-ß. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.

Caution

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Components

Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains.

Packaging

0.5 mg in glass bottle

Preparation Note

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 µm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.