Application

Immunohistochemical Analysis: A 1:50 dilution of this antibody detected SnoN in human skeletal muscle myocytes. Immunohistochemical Analysis: A 1:50 dilution of this antibody detected SnoN in human lung tissue.
Immunocytochemicaly Analysis: A 1:100 dilution of this antibody detected SnoN in H9 human embryonic stem cells.

This Anti-SnoN Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of SnoN.

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Ski family of oncogenes includes Ski and Sno (Ski-related novel gene, or Ski-like). Ski family members are nuclear proteins that form homodimers and heterodimers, bind to DNA and function as transcriptional activators and repressors. The Ski proteins regulate TGF induced gene-specific transcriptional activation by effectively repressing Smad activity and, thereby, inhibit TGF induced cell growth and extracellular matrix production. Recently, SnoN has been shown to maintain pluripotency through selective repression of mesendodermal genes in human ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to amino acids 358-372 of human SnoN protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in a human placenta tissue lysate. Western Blot Analysis: 1 µg/ml of this antibody detected SnoN in a 10µg of human placenta tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~80 kDa observed