Analysis Note

ControlSK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinomatesticular teratoma) cell lysate

Application

Research CategoryStem Cell Research

Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.

Research Sub CategoryHematopoietic Stem CellsNeural Stem Cells

Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.

Immunohistochemistry: 1:1,000 using the avidin-biotin system.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

Immunogen

Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Specificity

Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots (not less than 10 µL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Target description

80 kDa