Application

This Anti-mGCM1 Antibody is validated for use in WB for the detection of mGCM1.

Research CategoryStem Cell Research

Research Sub CategoryNeural Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GCM1 is a DNA binding protein with a GCM motif (glial cell missing motif) that is a key transcription factor for determining the fate of stem cells within the nervous system, in addition to being critical for placental development. This protein binds to the GCM motif (A/G)CCCGCAT, a novel sequence among known targets of DNA binding proteins. The N-terminal DNA-binding domain confers the unique DNA binding activity of this protein. Current studies indicate that the introduction of the mouse GCM1 gene in cultured cells from embryonic brains resulted in the induction of an astrocyte lineage.

Immunogen

Epitope: C-terminus

Synthetic GCM1 peptide, a.a. 401-418.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal antibody in buffer containing PBS with 0.09% sodium azide.

Peptide affinity purified

Quality

Routinely evaluated by western blot on HEK-293 lysates.

Western Blotting: Recommended working dilutions are 1:1000 – 1:2500.

Specificity

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from the C-terminal region mouse GCM1.

Comparison of sequence homology suggests that this antibody will also react in mouse and rat; however, reactivity to other species has not been tested.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Target description

50 kDa