Application

Immunoblotting (1 µg/ml)r>Immunocytochemistry (5-10 µg/ml)r>Immunoprecipitation (10 µg/ml)r>Paraffin Sections (whole mount) (see application references)

General description

Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.

PGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.

This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.

Protein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.

Immunogen

a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α

Mouse

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lai, L., et al. 2008. Genes Dev. 14, 1948.Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.

Endogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.

Packaging

1 set in Plastic ampoule

Physical form

One vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)