Analysis Note

ControlBrain tissue

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.

Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.

Immunogen

Epitope: a.a.420-445

Bovine brain kinesin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 µg of Mouse Brain lysates.

Specificity

Reacts with the heavy chain of kinesin near the N-terminus.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Target description

124 kDa