Analysis Note

ControlPRMT1 treated and untreated MEF cell lysates

Application

Detect dimethyl-Arginine using this Anti-dimethyl-Arginine Antibody, asymmetric (ASYM25) validated for use in WB.

General description

Asymmetrical dimethlarginine (ADMA) is an endogenous, L-arginine metabolite arising from the proteolysis of methylated arginine containing proteins and is a known inhibitor of nitric oxide synthase (NOS). In various studies, ADMA has been observed as having a regulatory role in cell motility and the actin cytoskeleton. Elevated levels of ADMA are associated with sporadic FSGS, coronary artery disease, hypertension, peripheral arterial occlusive disease, obesity, stroke, preeclampsia, and cardiovascular mortality/morbidity in patients with diabetic nephropathy.

Immunogen

KLH-conjugated linear peptide containing asymmetric dimethyl-arginine-glycine repeats.

Quality

Evaluated by Western Blot in PRMT1 treated and untreated MEF cell lysates.

Western Blot Analysis: 1:1,000 dilution of this antibody detected dimethyl-Arginine on 10 µg of PRMT1 treated and untreated MEF cell lysates.

Specificity

This antibody recognizes dimethylated arginines.

Target description

Multiple. This antibody detects proteins containing asymmetrical dimethylated arginines.