Analysis Note

ControlChick embryo fibroblast (CEF) cells expressing human wild-type Src protein

Application

Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.

Research CategorySignaling

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified rabbit polyclonal IgG in PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.

ImmunoAffinity Purified

Quality

Routinely evaluated by immunoblot.

Specificity

Recognizes Src phosphorylated on Tyrosine residue 418.

Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

60 kDa