Application

anti-phospho-IRS1 (Ser616) Antibody is an antibody against phospho-IRS1 (Ser616) for use in IC & WB.

Research CategorySignaling

Immunoblot Analysis: 0.5 µg/mL dilutions of this antibody detected phospho-IRS1 (Ser616) in RIPA lysates from TPA treated CHO cells transfected with human IRS1.

Research Sub CategoryInsulin/Energy Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified

100 µL of affinity purified rabbit polyclonal IgG in PBS (without Mg²⁺ and Ca²⁺), pH 7.3, containing 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide and 50% glycerol.

Quality

Evaluated by immunoblot.

Specificity

Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.

Human IRS1 (Insulin Receptor Substrate 1) phosphorylated on Serine residue 616. Sequence 100% homologous with mouse, rat, and pig.

Storage and Stability

1 year at -20°C.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.

Target description

165 kDa