Application

Immunoblot Analysis: A 1:1,000 dilution of this lot detected phospho-IR/IGF1R (Tyr1158) in RIPA lysate of untreated or insulin treated CHO cells transfected with IR.

Research CategorySignaling

Research Sub CategoryInsulin/Energy Signaling

Detect phospho-IR/IGF1R (Tyr1158) using this Anti-phospho-IR/IGF1R (Tyr1158) Antibody validated for use in IC, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

diabetes, insulin signaling, glucose

Immunogen

Peptide corresponding to the amino acid region encompassing the human, mouse, and rat phospho-Insulin Receptor (IR) Tyrosine 1158 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified

100 µL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

Specificity

Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.

Human phospho-Insulin Receptor (IR) Tyrosine 1158 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131. Mouse and rat reactivity are expected based on 100% sequence homology.

Storage and Stability

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Target description

155 kDa