Analysis Note

ControlHoxa9 immortalized HF1 cells

Application

Research Sub CategoryTranscription Factors

Detect Hox A9 with Anti-Hox A9 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in EMSA, WB, ICC.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa-9 markedly expands hematopoietic stem cells. HOXA9 expression changes dramatically with age - a uniformly low level of expression during early adulthood is replaced by a frequently very high expression in adults over sixty.

Immunogen

GST fusion protein corresponding to residues 194-272 of mouse Hox A9

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified immunoglobulin presented in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.

Protein A chromatography

Format: Purified

Quality

Routinely evaluated by immunoblot on whole cell lysates of Hoxa9 immortalized HF1 myeloid progenitor cells

Specificity

Hox A9

Based on a BLAST search, the antibody will likely cross react with Newt, Coelacanth, Dog, Chicken, and Rat Hox A9; Antelope, Mouse and Guinea Pig Hox 1.7; Frog, human and Zebrafish Hox A-9B; and Kenyan Clawed Frog Hox B.1

Storage and Stability

2 years at -20°C

Target description

36kDa