Analysis Note

ControlUntreated and TGFb1 treated NRK cell lysates

Application

Research CategorySignaling

Anti-Connective Tissue Growth Factor Antibody detects level of Connective Tissue Growth Factor & has been published & validated for use in WB.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Connective Tissue Growth Factor (CTGF) is a member of the CNN family of growth factors that are characterized as having cysteine-rich peptides with four specific domains. CTGF was originally identified in endothelial cells as a significant chemotactic and mitogenic factor. Research has revealed CTGF to have several biological properties which include chemotaxis, cell proliferation, promotion of adhesion and the formation of extracellular matrix. It is also necessary for such biological processes as wound healing, scar formation, fibrosis, embryogenesis and angiogenesis. In its role as an inducer of angiogenesis, CTGF has been associated with vascular endothelial growth factor (VEGF). As a result of its pro-fibrotic properties, CTGF is thought to be a promising target for therapy in conditions involving pathological fibrosis such as chronic fibrotic disease, chronic kidney disease and proliferative diabetic retinopathy (PDR). Cell types found to express CTGF are endothelial cells, fibroblasts and vascular smooth-muscle cells.

Immunogen

Epitope: Mature chain

Recombinant protein corresponding to the mature chain of human Connective tissue growth factor.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified goat polyclonal in buffer containing Tris-Glycine pH 8.0 with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in untreated and TGFb1 treated NRK cell lysates.

Western Blot Analysis: 5 µg/mL of this antibody detected Connective tissue growth factor on 10 µg of untreated and TGFb1 treated NRK cell lysates.

Specificity

This antibody recognizes the mature chain of Connective tissue growth factor.

Primate (98% sequence homolgy). Monkey (97% sequence homology). Porcine and Bovine (93% sequence homology). Rat and mouse (91% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

In Western Blot, full length CTGF is observed at 38 kDa and CTGF N&C domain is observed at 19 kDa