Analysis Note

ControlPMA treated and untreated MOLT4 cell lysates

Application

Research CategoryEpigenetics & Nuclear FunctionSignaling

Research Sub CategoryTranscription Factors

Detect phospho-STAT3 (Tyr68) using this Anti-phospho-STAT3 (Tyr68) Antibody validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element).

Immunogen

Epitope: Phosphorylated Tyr68

KLH-conjugated linear peptide corresponding to human STAT3 phosphorylated at Tyr68.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity

Quality

Evaluated by Western Blot in PMA treated and untreated MOLT4 cell lysates.

Western Blot Analysis: 5 µg/mL of this antibody detected STAT3 on 10 µg of PMA treated and untreated MOLT4 cell lysates.

Specificity

This antibody recognizes STAT3 phosphorylated at Tyr68.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed