Analysis Note

ControlHEK293T cells transfected with myc-tagged Src and CBP

Application

Research Sub CategoryCytoskeleton

Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.

Research CategoryCell Structure

This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Function Assay for the detection of acetylated-Src (Lys430).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.

Immunogen

Epitope: Acetylated Lys430

KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.

Specificity

This antibody recognizes Src acetylated at Lys430.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.