Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Sin3A in HeLa, A431, HUVEC, and NIH/3T3 cells.

Anti-Sin3A Antibody is an antibody against Sin3A Antibody for use in Western Blotting and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Paired amphipathic helix protein Sin3a (UniProt Q60520; also known as Histone deacetylase complex subunit Sin3a, mSin3a, Transcriptional co-repressor Sin3a) is encoded by the Sin3A (also known as mSin3A, AW553200, Kiaa4126, mKIAA4126) gene (Gene ID 8819) in murine species. The transcription regulator Sin3a is a scaffold protein that interacts with various proteins to form specialized complexes that mediate different biological processes, including chromatin modification, embryonic development, DNA repair, mitochondrial metabolism, and gene transcription. SIN3a harbors four paired α-helices (PAH), a histone interaction domain, and a highly conserved region. The histone interaction domain mediates HDACs binding, whereas the highly conserved region interacts with proteins necessary for repression, such as the nuclear receptor hormone co-repressor Alien. The four PAHs represent the main interacting domains of SIN3a and mediate the interactions with a wide variety of DNA-binding transcription factors. Members of the Mad family of repressor proteins, for example, specifically interact with the PAH2 through their Sin3 interacting domain (SID). Although widely studied for its involvement in transcriptional repression, Sin3a is shown to be essential for aryl hydrocarbon receptor-mediated induction of gene transcription.

Immunogen

Epitope: N-terminus

KLH-conjugated peptide (KRRLDDQESPVYAAQQRR-C) corresponding to amino acids 2-19 of mouse Sin3A.

Linkage

Replaces: 06-913

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Sin3A in 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 kDa observed