Analysis Note

ControlA431 cell lysate
Included Positive Antigen Control: Catalog #12-301, Non stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Application

Research Sub CategoryTranscription Factors

Use Anti-β-Catenin Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD).

Immunoprecipitation:
5 µg of a previous lot immunoprecipitated β-Catenin from 500 µg of A431 membrane fraction lysate.

Immunocytochemistry:
1-5 µg/mL of a previous lot showed positive immunostaining for β-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton^™ X-100.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected beta-Catenin in A431 and HUVEC cell lines.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

β-catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors.
β-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. β-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated β-catenin affects the protein association of β-catenin to E-cadherin and α-cateinin that occurs in the cell. This process can be reversed by de-phosphorylation of β-catenin.

Immunogen

KLH-conjugated, synthetic peptide (CGG-SYLDSGIHSGATTTAPSLSGK) corresponding to the consensus GSK3 phosphorylation site of human β -Catenin (amino acids 29-49).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A chromatography

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Quality

Routinely evaluated by western blot on A431 total cell lysate.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected β-catenin in an A431 total cell lysate.

Specificity

Recognizes β-Catenin, Mr 92 kDa.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution.

Target description

92 kDa