Application

Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB.

General description

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Immunogen

An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-240

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract

Specificity

Recognizes Kip1.

Target description

27 kDa