Analysis Note

ControlIncluded Negative Control: Catalog # 16-239, Alexa Fluor^® 555-conjugated Normal Mouse IgG.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.

Disclaimer

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

25µg Alexa Fluor^® 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween™-20, 0.05% sodium azide. Liquid at 4°C.

Protein G Purified

Quality

Immunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40µg of rat brain microsomal preparation.

Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.

Flow Cytometry: 0.2µg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).

Specificity

Specific for the α-1 subunit isoform.

Storage and Stability

Do Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.

Target description

113 kDa