Analysis Note
ControlHuman placental villi and tonsil tissue
Application
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Soos, M., et al. (1992). Journal of Biological Chemistry. 267(18):12955-12963.)
Research Sub CategoryInsulin/Energy Signaling
Research CategoryMetabolism
This Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Immunogen
Epitope: Unknown
KLH-conjugated linear peptide corresponding to human IGF-IR.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Protein G Purified
Format: Purified
Quality
Evaluated by Immunohistochemistry in human placental villi and tonsil tissue.
Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected IGF-1R in human placental villi and tonsil tissue.
Specificity
This antibody recognizes IGF-IR.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
155 kDa
This product has met the following criteria: