Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding ProteinsRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SMN in HeLa cells.

Anti-SMN Antibody, clone 2B1 is a Mouse Monoclonal Antibody for detection of SMN also known as Spinal muscular atrophy (Werdnig-Hoffmann disease Kugelberg-Welander disease) & has been validated in WB, ICC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The survival of motor neurons (SMN) protein is essential for the biogenesis of small nuclear RNA (snRNA)-ribonucleoproteins (snRNPs), the major components of the pre-mRNA splicing machinery. Though it is ubiquitously expressed, SMN deficiency causes the motor neuron degenerative disease spinal muscular atrophy (SMA). SMN deficiency has unexpected cell type-specific effects on the repertoire of snRNAs and mRNAs. It alters the stoichiometry of snRNAs and causes widespread pre-mRNA splicing defects in numerous transcripts of diverse genes, preferentially those containing a large number of introns, in SMN-deficient mouse tissues. The SMN complex plays a role in RNA metabolism and in splicing regulation.

Immunogen

Epitope: Unknown

His-tagged recombinant protein corresponding to human SMN.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected SMN on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes SMN.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

35 kDa was observed; however, the calculated molecular weight is 31.849 kDa