Imidazole, BioUltra, >=99.5% (GC)

Code: 56749-250G D2-231

Application

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase...


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Application

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Excellent for buffers in the range of pH 6.2-7.8

Other Notes

Buffer for assay of horseradish peroxidase

λ0.1 M in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤50 mg/kg
assay≥99.5% (GC)
bp256 °C (lit.)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Ca: ≤10 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, Pb: ≤5 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Cd: ≤5 mg/kg, Sr: ≤5 mg/kg, Co: ≤5 mg/kg, Mg: ≤5 mg/kg
formpowder or crystals
ign. residue (900 °C)≤0.05%
impuritiesinsoluble matter, passes filter test, ≤0.2% water
InChI keyRAXXELZNTBOGNW-UHFFFAOYSA-N
InChI1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
mp88-91 °C (lit.)
pH9.5-11.0 (25 °C, 0.1 M in H2O)
pKa (25 °C)6.95
product lineBioUltra
Quality Level200
SMILES stringc1c[nH]cn1
solubilityH2O: 0.1 M at 20 °C, clear, colorless
UV absorptionλ: 260 nm Amax: 0.10, λ: 280 nm Amax: 0.10
vapor pressure﹤1 mmHg ( 20 °C)
Code
Description
Unit Size
List Price
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56749-1KG
Imidazole, BioUltra, >=99.5% (GC)

Unit:1KG
List Price: £801.00
Source:List Price
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56749-50G
Imidazole, BioUltra, >=99.5% (GC)

Unit:50G
List Price: £64.60
Source:List Price
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Cas Number288-32-4
Hazard Class8
Un Number3263
Pack GroupIII
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