Biochem/physiol Actions

Primary Targetchymotrypsin

Product does not compete with ATP.

General description

Specific irreversible inhibitor of serine proteases. Reacts covalently with a serine at the active site. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. Stable, nontoxic alternative to PMSF (Cat. No. 52332) and DFP (Cat. No. 30967). Used at the same molar concentration (0.1-1.0 mM) as PMSF for most applications. Exhibits low inhibitory activity towards proteinase K.

Specific, irreversible inhibitor of serine proteases. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. Stable, nontoxic alternative to PMSF (Cat. No. 52332) and DFP (Cat. No. 30967). A β-secretase inhibitor that inhibits β-amyloid peptide (Aβ) production and enhances amyloid precursor protein (sAPPU_α) secretion in several cell lines at millimolar concentrations. Use at the same molar concentrations (0.1-1.0 mM) as PMSF for most applications. Also available as a 50 mM solution in H₂O (Cat. No. 508436).

AEBSF, CAS 30827-99-7, is a specific irreversible inhibitor of chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. A nontoxic alternative to PMSF and DFP.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Citron, M., et al. 1996. Neuron 17, 171.Ohkubo, I., et al. 1994. J. Biochem. 116, 1182.Mintz, G.R. 1993. BioPharm. 6, 34.Lawson, W.B., et al. 1982. Folia Haematol.109, 52.Markwardt, F., et al. 1974. Biochem. Pharmacol.23, 2247.Markwardt, F., et al. 1973. Thromb. Res.2, 343.Baker, B.R., and Cory, M. 1971. J. Med. Chem.14, 119.

Packaging

Packaged under inert gas

500 mg in Plastic ampoule

1 g in Glass bottle

50, 100 mg in Glass bottle

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 months at 4°C. Slight hydrolysis occurs at alkaline pH (8-9).

Warning

Toxicity: Standard Handling (A)