Application

Research CategoryApoptosis & Cancer

Detect Cathepsin B using this rabbit polyclonal Anti-Cathepsin B Antibody, Cat. No. 06-480-I. It has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin B in human cerebral cortex, human liver, human bone marrow, and human prostate cancer tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cathepsin B (UniProt: P00787; also known as Cathepsin B1, RSG-2) is encoded by the Ctsb gene (Gene ID: 64529) in rat. Cathepsin B is a secreted lysosomal a lysosomal cysteine protease protease that is synthesized as a procathepsin with a signal peptide of 17 amino acids that directs it the lumen of rough endoplasmic reticulum where the signal peptide is removed to generate the inactive procathepsin B with a propeptide sequence. Procathepsin B is then transported to the Golgi apparatus where it is glycosylated at two asparagine residues by mannose containing oligosaccharides with phosphorylated mannose residues. The propeptide functions as an inhibitor and also stabilizes the enzyme. Procathepsin B is transported to lysosomes where under acidic conditions it undergoes autocatalytic activation following the removal of propeptide. Active cathepsin B is a dimer of a light chain and a heavy chain cross-linked by a disulfide bond. Cathepsin B hydrolyses proteins with broad specificity and preferentially cleaves Arg-Arg-|-Xaa bonds. In addition to being an endopeptidase, it also shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. Cathepsin B expression is elevated in several human cancers at the mRNA, protein, and activity levels. It is also frequently overexpressed in premalignant lesions. During the transition to malignancy, cathepsin B localizes to the cell periphery and at the basal pole of polarized cells and active cathepsin B is secreted from tumors and facilitates in invasion and metastasis. (Ref.: Aggarwal, N and Sloane, BF (2014) Proteomics Clin. Appl. 8, 427-437).

Immunogen

GST-tagged recombinant fragment of 322 amino acids from rat cathepsin B.

Linkage

Replaces: 06-480

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in rat kidney microsomal preparations

Western Blotting Analysis: 2 µg/mL of this antibody detected Cathepsin B in 10 µg of rat kidney microsomal preparartion.

Specificity

This polyclonal antibody specifically recognizes Cathepsin B. It detects both pro and mature forms of cathepsin B.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed; 37.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).